The Role of Phytohormones in the Induction of Somatic Embryogenesis in <i>Pinus sibirica</i> and <i>Larix sibirica</i>

The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte embryo), proliferating embryonal-suspensor mass (ESM), non-embryogenic callus Pinus sibirica Larix sibirica. Furthermore, the localization hormones cells was studied with immunohistochemical analysis. It shown that formation embryogenic cultures both species is associated content phytohormones their cells. In L. IAA ESM about 100 times higher than calli. At same time, a low ABA characteristic cultures. Non-embryogenic contains an increased cytokinins ABA. transition somatic to path development characterized by elongation cells, asymmetric division, at one ends elongated consisted isodiametric, actively dividing